Description

  • Product name

    Recombinant human PDE7A/HCP1 protein
    See all PDE7A/HCP1 proteins and peptides
  • Biological activity

    The specific activity of ab125786 was determined to be 166 nmol/min/mg.
  • Purity

    > 85 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSSNSL NILDDDYNGQ AKCMLEKVGN WNFDIFLFDR LTNGNSLVSL TFHLFSLHGL IEYFHLDMMK LRRFLVMIQE DYHSQNPYHN AVHAADVTQA MHCYLKEPKL ANSVTPWDIL LSLIAAATHD LDHPGVNQPF LIKTNHYLAT LYKNTSVLEN HHWRSAVGLL RESGLFSHLP LESRQQMETQ IGALILATDI SRQNEYLSLF RSHLDRGDLC LEDTRHRHLV LQMALKCADI CNPCRTWELS KQWSEKVTEE FFHQGDIEKK YHLGVSPLCD RHTESIANIQ IGFMTYLVEP LFTEWARFSN TRLSQTMLGH VGLNKASWKG LQREQSSSED TDAAFELNSQ LLPQENRLS
    • Predicted molecular weight

      64 kDa including tags
    • Amino acids

      104 to 456
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as PDE7A

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.4% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 5''-cyclic phosphodiesterase 7A
    • HCP 1
    • HCP1
    • High affinity cAMP specific 3'5' cyclic phosphodiesterase 7A
    • High affinity cAMP-specific 3''
    • P2A
    • PDE 7
    • PDE 7A
    • PDE1/PDE2 yeast human complement of
    • PDE7
    • PDE71
    • PDE7A
    • PDE7A_HUMAN
    • Phosphodiesterase 7A
    • Phosphodiesterase isozyme 7
    • Phosphodiesterase7A
    • Rolipram insensitive phosphodiesterase type 7
    • TM 22
    • TM22
    see all
  • Function

    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction.
  • Tissue specificity

    PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart.
  • Pathway

    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.
  • Developmental stage

    Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle.
  • Domain

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Cellular localization

    Cytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions.
  • Information by UniProt

Images

  • The specific activity of PDE7A/HCP1 (ab125786) was determined to be 160 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab125786
  • SDS PAGE analysis of ab125786
  • SDS-PAGE analysis of ab125786.
  • Sample assay showing the specific activity of ab125786 to be 166 nmol/min/mg.

References

ab125786 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab125786.
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