The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 97 U/ug. 1 unit will release 1 pmole phosphate per minute from 400 uM 5’-GMP at 30 °C in a reaction buffer of 10 mM Tris-HCl, pH 7.4, 1 mM MgCl2. Assay buffer: 10mM Tris-HCl, pH 7.4, 1 mM Magnesium chloride ; 3’, 5’-cGMP substrate: 0.4 mM; PDE9A: 5 ng/ul.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.