Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Phosphatase Activity, Inhibition Assay
Product nameRecombinant human PDE9A protein
See all PDE9A proteins and peptides
Biological activitySpecific Activity: 97 U/ug. 1 unit will release 1 pmole phosphate per minute from 400 uM 5’-GMP at 30 °C in a reaction buffer of 10 mM Tris-HCl, pH 7.4, 1 mM MgCl2. Assay buffer: 10mM Tris-HCl, pH 7.4, 1 mM Magnesium chloride ; 3’, 5’-cGMP substrate: 0.4 mM; PDE9A: 5 ng/ul.
Purity> 75 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab54113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1535% Glutathione, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase 9A
- cGMP specific 3' 5' cyclic phosphodiesterase type 9
FunctionHydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
Tissue specificityExpressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.
DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Cellular localizationCytoplasm. Endoplasmic reticulum; Cell projection > ruffle membrane. Cytoplasm > perinuclear region and Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Golgi apparatus. Endoplasmic reticulum.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab54113 has not yet been referenced specifically in any publications.