Recombinant human PDGF AA protein (Animal Free) (ab179505)

Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
    • Molecular weight

      29 kDa
    • Amino acids

      87 to 211
    • Additional sequence information

      ab179505 is a non-glycosylated disulfide-linked homodimer, containing two 125 amino acid chains.

Specifications

Our Abpromise guarantee covers the use of ab179505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically 3-5 ng/mL.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level

    <=1.000 Eu/µg
  • Purity

    > 97 % SDS-PAGE.

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

General Info

  • Alternative names

    • PDGF A
    • PDGF A chain
    • PDGF subunit A
    • PDGF-1
    • PDGF1
    • PDGFA
    • PDGFA_HUMAN
    • Platelet derived growth factor alpha chain
    • Platelet derived growth factor alpha isoform 2 preproprotein
    • Platelet derived growth factor alpha polypeptide
    • Platelet-derived growth factor A chain
    • Platelet-derived growth factor alpha polypeptide
    • Platelet-derived growth factor subunit A
    see all
  • Function

    Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
  • Domain

    The long form contains a basic insert which acts as a cell retention signal.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab179505 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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