Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PDGF AB protein
See all PDGF AB proteins and peptides
Purity> 95 % SDS-PAGE.
Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng per µg.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab52112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesThe lyophilized protein is best-stored desiccated below 0oC. Reconstituted human PDGF-AB should be stored in working aliquots at -20oC. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or –20oC for future use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: 0.06% Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml.
RelevancePlatelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52112 has not yet been referenced specifically in any publications.