Bioactive grade

Recombinant human PDGF AB protein (Animal Free) (ab179496)

Description

  • Product name

    Recombinant human PDGF AB protein (Animal Free)
    See all PDGF AB proteins and peptides
  • Biological activity

    The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.

  • Purity

    > 95 % SDS-PAGE.
    Purity determined by Reducing and Non-reducing SDS-PAGE.
  • Endotoxin level

    <=1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      Alpha chain: MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVK RCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLEC ACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT Beta chain: SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPP CVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIGIVRKKPIFKKATVTLG DHLACKCETVAAARPVT
    • Predicted molecular weight

      26 kDa
    • Additional sequence information

      Non-glycosylated, disulfide-linked heterodimer, containing one 14.3 kDa alpha-chain (aa87-211; UniProt P04085) and one 12.1 kDa beta-chain (aa82-190; UniProt P01127).

Specifications

Our Abpromise guarantee covers the use of ab179496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilised product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

General Info

  • Alternative names

    • becaplermin
    • c-sis
    • GDGF
    • ODGF
    • Oncogene SIS
    • PDGF A chain
    • PDGF B chain
    • PDGF subunit A
    • PDGF subunit B
    • PDGF1
    • PDGF2
    • PDGFA
    • PDGFB
    • Platelet derived growth factor 2
    • Platelet derived growth factor A chain
    • Platelet derived growth factor alpha chain
    • Platelet derived growth factor alpha polypeptide
    • Platelet derived growth factor subunit A
    • platelet-derived growth factor 2
    • platelet-derived growth factor A chain
    • platelet-derived growth factor alpha chain
    • platelet-derived growth factor alpha isoform 2 preproprotein
    • platelet-derived growth factor alpha polypeptide
    • platelet-derived growth factor B chain
    • platelet-derived growth factor beta polypeptide
    • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
    • platelet-derived growth factor subunit A
    • platelet-derived growth factor subunit B
    • platelet-derived growth factor, beta polypeptide (oncogene SIS)
    • proto-oncogene c-Sis
    • Simian sarcoma viral oncogene homolog
    • SIS
    • SSV
    • V-SIS platelet-derived growth factor beta polypeptide
    see all
  • Relevance

    Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
  • Cellular localization

    Secreted

Images

  • Functional analysis of ab179496
  •  SDS PAGE analysis of ab179496 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

References

ab179496 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab179496.
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