Recombinant human PDGFR beta protein (ab60833)
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Overview
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Product nameRecombinant human PDGFR beta protein
See all PDGFR beta proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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Molecular weight104 kDa
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Amino acids557 to 1106
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TagsGST tag N-Terminus
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Additional sequence informationRecombinant fragment, corresponding to amino acids 557-end of Human PDGF Receptor beta.
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activity
Specific Activity: 20 nmol/min/mg.
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Applications
Functional Studies
SDS-PAGE
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Purity> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. -
FormLiquid
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Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Beta platelet derived growth factor receptor
- Beta-type platelet-derived growth factor receptor
- CD 140B
see all -
FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
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Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
Cellular localizationMembrane.
- Information by UniProt
Images
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Functional Studies - Recombinant human PDGFR beta protein (ab60833)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PDGFR beta protein (ab60833)ab60833 on SDS-PAGE, MW ~104kDa.
Datasheets and documents
References
ab60833 has not yet been referenced specifically in any publications.
