Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Product nameRecombinant human PDGFR beta protein (Active)
See all PDGFR beta proteins and peptides
Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-BB, Tag Free at 2 μg/mL (100 μL/well) can bind Human PDGFRB, His Tag (ab155703) with a linear range of 0.039-0.625 μg/mL
Purity> 95 % SDS-PAGE.
Lyophilized from 0.22 µm filtered solution
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceLVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFS SVLTLTNLTG LDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPN DAEELFIFLTEITEITIPCRVT DPQLVVTLHEKKGDVALPVPYDHQRG FSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVS SINVSVNAVQTVVR QGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHI RS ILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGT LQFAELHR SRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNV SETRYVSELTLVRVKVAEAG HYTMRAFHEDAEVQLSFQLQINVPVRVL ELSESHPDSGEQTVRCRGRGMPQPNIIWSACR DLKRCPRELPPTLLGN SSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRN AVGQ DTQEVIVVPHSLPF
Predicted molecular weight57 kDa including tags
Amino acids33 to 530
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab155703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 95% PBS, 5% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 500 µg/ml.
- Beta platelet derived growth factor receptor
- Beta-type platelet-derived growth factor receptor
- CD 140B
FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
- Information by UniProt
The purity of ab155703 was determined by SDS-PAGE of reduced ab155703 and stained overnight with Coomassie Blue. The protein migrates as 95-100 kDa due to glycosylation.
Immobilized Human PDGF-BB, Tag Free at 2 μg/mL (100 μL/well) can bind Human PDGFRB, His Tag (ab155703) with a linear range of 0.039-0.625 μg/mL.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab155703 has not yet been referenced specifically in any publications.