Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human PDK3 protein
The specific activity of ab125606 was determined to be 12 nmol/mg/min.
Purity> 90 % Densitometry.
Purity determined to be >90% by densitometry. Affinity purified. Purity is lot specific. Please contact our technical Support team for details.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight69 kDa including tags
Amino acids1 to 406
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab125606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3
FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Tissue specificityFound almost exclusively in heart and skeletal muscle.
Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125606 has not yet been referenced specifically in any publications.