Recombinant human PDK4 protein (ab125560)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PDK4 protein
See all PDK4 proteins and peptides -
Biological activity
The specific activity of ab125560 is 20 nmol/min/mg. -
Purity
> 90 % Densitometry.
ab125560 is >90% pure. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
71 kDa -
Amino acids
1 to 411 -
Tags
GST tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.004% DTT, 0.002% PMSF, 0.31% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
- FLJ40832
- mitochondrial
see all -
Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Ubiquitous; highest levels of expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125560 has not yet been referenced specifically in any publications.