Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      Q16654
    • Species
      Human
    • Molecular weight
      71 kDa
    • Amino acids
      1 to 411
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125560 is 20 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 90 % Densitometry.
    ab125560 is >90% pure.
  • Form
    Liquid
  • Additional notes

    ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
    • FLJ40832
    • mitochondrial
    • Pdk4
    • PDK4_HUMAN
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial
    • Pyruvate dehydrogenase kinase 4
    • Pyruvate dehydrogenase kinase isoenzyme 4
    • Pyruvate dehydrogenase kinase isoform 4
    • Pyruvate dehydrogenase kinase isozyme 4
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial
    see all
  • Function
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity
    Ubiquitous; highest levels of expression in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PDK4 protein (ab125560)
    Functional Studies - Recombinant human PDK4 protein (ab125560)
    The specific activity of ab125560 was determined to be 20 nmol/min/mg.
  • SDS-PAGE - Recombinant human PDK4 protein (ab125560)
    SDS-PAGE - Recombinant human PDK4 protein (ab125560)
    SDS PAGE analysis of ab125560 (PDK4 protein).

References

ab125560 has not yet been referenced specifically in any publications.

Publishing research using ab125560? Please let us know so that we can cite the reference in this datasheet.

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