Recombinant human PDK4 protein (ab125560)

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Functional Studies - Recombinant human PDK4 protein (ab125560)
  • SDS-PAGE - Recombinant human PDK4 protein (ab125560)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: WB, Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human PDK4 protein
    See all PDK4 proteins and peptides

  • Biological activity

    The specific activity of ab125560 is 20 nmol/min/mg.

  • Purity

    > 90 % Densitometry.
    ab125560 is >90% pure.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q16654

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      71 kDa

    • Amino acids

      1 to 411

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.004% DTT, 0.002% PMSF, 0.31% Glutathione

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
    • FLJ40832
    • mitochondrial
    • Pdk4
    • PDK4_HUMAN
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial
    • Pyruvate dehydrogenase kinase 4
    • Pyruvate dehydrogenase kinase isoenzyme 4
    • Pyruvate dehydrogenase kinase isoform 4
    • Pyruvate dehydrogenase kinase isozyme 4
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial
    see all

  • Function

    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

  • Tissue specificity

    Ubiquitous; highest levels of expression in heart and skeletal muscle.

  • Sequence similarities

    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PDK4 protein (ab125560)
    Functional Studies - Recombinant human PDK4 protein (ab125560)
    The specific activity of ab125560 was determined to be 20 nmol/min/mg.
  • SDS-PAGE - Recombinant human PDK4 protein (ab125560)
    SDS-PAGE - Recombinant human PDK4 protein (ab125560)
    SDS PAGE analysis of ab125560 (PDK4 protein).

References (0)

Publishing research using ab125560? Please let us know so that we can cite the reference in this datasheet.

ab125560 has not yet been referenced specifically in any publications.

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