Description

  • Product name

    Recombinant Human PDP1/PDP protein
  • Purity

    > 90 % Purified via His tag.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      72 to 537

Specifications

Our Abpromise guarantee covers the use of ab110357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Sandwich ELISA

  • Form

    Liquid
  • Additional notes

    The pyruvate dehydrogenase complex (PDH) is at the center of aerobic carbohydrate metabolism. It is localized in the matrix space of mitochondria where it catalyzes the irreversible oxidative decarboxylation of pyruvate entering the organelle to produce acetyl-CoA, NADH and CO2. PDH is a complex of three different enzymes that channel substrate and product. These are pyruvate dehydrogenase (E1), dihydrolipoamide transacetylase (E2) and dihydrolipoamide dehydrogenase (E3). PDH deficiencies, diabetes, starvation, sepsis, and possibly Alzheimer's disease have been linked to altered PDH functioning.

    Product was previously marketed under the MitoSciences sub-brand.

     This product was previously labelled as PDP1

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100

General Info

  • Alternative names

    • [Pyruvate dehydrogenase [acetyl transferring]] phosphatase 1, mitochondrial
    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1
    • FLJ32517
    • Gm1024
    • MGC119646
    • mitochondrial
    • PDH
    • PDP
    • PDP 1
    • Pdp1
    • PDP1_HUMAN
    • PDPC
    • PDPC 1
    • PPM2C
    • Protein phosphatase 2C
    • protein phosphatase 2C, magnesium dependent, catalytic subunit
    • Pyruvate dehydrogenase (Lipoamide) phosphatase phosphatase
    • Pyruvate dehydrogenase phosphatase catalytic subunit 1
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 1
    • RP23 203A12.1
    see all
  • Function

    Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • Involvement in disease

    Defects in PDP1 are the cause of pyruvate dehydrogenase phosphatase deficiency (PDP deficiency) [MIM:608782]. PDP deficiency results in lactic acidosis leading to neurological dysfunction.
  • Sequence similarities

    Belongs to the PP2C family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

References

ab110357 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We advise use of PBS for homogenization of muscle tissue. We have not tested the use of this kit with fat tissue. If you have a favorite homogenization buffer, we would advice to prepare the homogenates of both tissues in both buffers in parallel , run the assay and compare the results. Positive control samples are available (Bovine Heart Mitochondrial lysate (ab110338)). Regarding the PDP1 and PDP2, samples can be dephosphorylated with these enzymes prior or after the immunocapture in the ab109902 plate. The dephosphorylation using PDP1 after the immunocapture (in well) is described in the Modification section of the this protocol, for more information also see the protocol figures: http://www.mitosciences.com/PDF/endogenous_pdh_activity_phosphorylated_dephosphorylated_states.pdf PDP2 can be used in a similar fashion, in this case supplement the dephosphorylation buffer with 10 mM MgCl2 instead of CaCl2. Use PDP2 at 4 ug/well.

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