Recombinant Human PER2 protein (ab112382)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human PER2 protein -
Biological activity
Useful for Antibody Production and Protein Array -
Expression system
Wheat germ -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MNGYAEFPPSPSNPTKEPVEPQPSQVPLQEDVDMSSGSSGHETNENCSTG RDSQGSDCDDSGKELGMLVEPPDARQSPDTFSLMMAKSEHNPSTSGCSSD -
Predicted molecular weight
37 kDa including tags -
Amino acids
1 to 100
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Specifications
Our Abpromise guarantee covers the use of ab112382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
ab112382 is best used within three months from the date of receiptUseful for Antibody Production and Protein Array -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Note: Glutathione is reduced
General Info
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Alternative names
- Circadian clock protein PERIOD 2
- FASPS
- FASPS1
see all -
Function
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
NPAS2-BMAL1
BMAL2-induced transactivation. -
Tissue specificity
Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung. -
Involvement in disease
Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
Sequence similarities
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsPhosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PER2 protein (ab112382)ab112382 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.
Datasheets and documents
References
ab112382 has not yet been referenced specifically in any publications.
