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Recombinant human Peroxiredoxin 1/PAG protein (ab79945)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
  • Functional Studies - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
  • Western blot - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)

Key features and details

  • Expression system: Baculovirus
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Suitable for: WB, Functional Studies, SDS-PAGE

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Human Peroxiredoxin 1 ELISA Kit (PRX1) (ab185983)

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Description

  • Product name

    Recombinant human Peroxiredoxin 1/PAG protein
    See all Peroxiredoxin 1/PAG proteins and peptides
  • Biological activity

    Specific Activity between 800 and 1000 pmol/min/µg. Please enquire as to activity of specific lots. 1.88 µg of Peroxiredoxin 1 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
  • Purity

    > 90 % SDS-PAGE.
    Affinity purified.
  • Expression system

    Baculovirus
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

  • Related Products

    • Anti-Peroxiredoxin 1/PAG antibody (ab15571)
    • Anti-Peroxiredoxin 1/PAG antibody (ab41906)
    • Anti-Peroxiredoxin 1/PAG antibody (ab59538)

Specifications

Our Abpromise guarantee covers the use of ab79945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Heme binding 23 kDa protein
    • MSP23
    • Natural killer cell-enhancing factor A
    • NKEF A
    • NKEF-A
    • NKEFA
    • OSF3
    • Osteoblast specific factor 3
    • PAG
    • Paga
    • PAGB
    • Peroxiredoxin 1
    • Peroxiredoxin-1
    • PRDX1
    • PRDX1_HUMAN
    • Proliferation associated gene A
    • Proliferation-associated gene protein
    • PRX1
    • PrxI
    • TDPX2
    • Thioredoxin peroxidase 2
    • Thioredoxin-dependent peroxide reductase 2
    see all
  • Function

    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • Sequence similarities

    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications

    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession Q06830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
    SDS-PAGE - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
    Lane 1: ab79945 on 14% SDS-PAGE, Coomassie staining, 20µg.
    Lane 2: Protein marker.
  • Functional Studies - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
    Functional Studies - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
  • Western blot - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
    Western blot - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
    This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab79945? Please let us know so that we can cite the reference in this datasheet.

ab79945 has been referenced in 1 publication.

  • Booty LM  et al. Selective Disruption of Mitochondrial Thiol Redox State in Cells and In Vivo. Cell Chem Biol 26:449-461.e8 (2019). PubMed: 30713096

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