Recombinant human Peroxiredoxin 1/PAG protein (ab79945)
Key features and details
- Expression system: Baculovirus
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Peroxiredoxin 1/PAG protein
See all Peroxiredoxin 1/PAG proteins and peptides -
Biological activity
Specific Activity between 800 and 1000 pmol/min/µg. Please enquire as to activity of specific lots. 1.88 µg of Peroxiredoxin 1 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min. -
Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab79945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Heme binding 23 kDa protein
- MSP23
- Natural killer cell-enhancing factor A
see all -
Function
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Post-translational
modificationsPhosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Images
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SDS-PAGE - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)Lane 1: ab79945 on 14% SDS-PAGE, Coomassie staining, 20µg.
Lane 2: Protein marker. -
Functional Studies - Recombinant human Peroxiredoxin 1/PAG protein (ab79945) -
Western blot - Recombinant human Peroxiredoxin 1/PAG protein (ab79945)This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab79945 has been referenced in 1 publication.
- Booty LM et al. Selective Disruption of Mitochondrial Thiol Redox State in Cells and In Vivo. Cell Chem Biol 26:449-461.e8 (2019). PubMed: 30713096