Recombinant Human PFTK1 protein (ab167928)

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SDS-PAGE - Recombinant Human PFTK1 protein (ab167928)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 95% Densitometry
    • Tags: proprietary tag N-Terminus
    • Suitable for: SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human PFTK1 protein

    • Purity

      > 95 % Densitometry.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      O94921-2

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MHGYFGCNAA AEPGYSAFVG TPQICVTKMS TRNCQGMDSV IKPLDTIPED KKVRVQRTQS TFDPFEKPAN QVKRVHSENN ACINFKTSST GKESPKVRRH SSPSSPTSPK FGKADSYEKL EKLGEGSYAT VYKGKSKVNG KLVALKVIRL QEEEGTPFTA IREASLLKGL KHANIVLLHD IIHTKETLTL VFEYVHTDLC QYMDKHPGGL HPDNVKLFLF QLLRGLSYIH QRYILHRDLK PQNLLISDTG ELKLADFGLA RAKSVPSHTY SNEVVTLWYR PPDVLLGSTE YSTCLDMWGV GCIFVEMIQG VAAFPGMKDI QDQLERIFLV LGTPNEDTWP GVHSLPHFKP ERFTLYSSKN LRQAWNKLSY VNHAEDLASK LLQCSPKNRL SAQAALSHEY FSDLPPRLWE LTDMSSIFTV PNVRLQPEAG ESMRAFGKNN SYGKSLSNSK H

      • Predicted molecular weight

        72 kDa including tags

      • Amino acids

        1 to 451

      • Tags

        proprietary tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab167928 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    General Info

    • Alternative names

      • cdk14
      • CDK14_HUMAN
      • Cell division protein kinase 14
      • Cyclin-dependent kinase 14
      • hPFTAIRE1
      • KIAA0834
      • mKIAA0834
      • PFTAIRE protein kinase 1
      • PFTAIRE1
      • PFTK1
      • Serine/threonine protein kinase PFTAIRE 1
      • Serine/threonine-protein kinase PFTAIRE-1
      see all

    • Function

      Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.

    • Tissue specificity

      Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected.

    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.

    • Cellular localization

      Cell membrane. Cytoplasm. Nucleus. Recruited to the cell membrane by CCNY.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PFTK1 protein (ab167928)
      SDS-PAGE - Recombinant Human PFTK1 protein (ab167928)
      SDS-PAGE analysis of ab167928.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab167928? Please let us know so that we can cite the reference in this datasheet.

    ab167928 has been referenced in 1 publication.

    • Lu F  et al. Upregulation of miR-1825 inhibits the progression of glioblastoma by suppressing CDK14 though Wnt/ß-catenin signaling pathway. World J Surg Oncol 18:147 (2020). PubMed: 32605563

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