Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      TGELRDSDFS NEQFSKLPMF INSGLAMDGL FDDSEDESDK LSYPWDGTQS YSLFNVSPSC SSFNSPCRDS VSPPKSLFSQ RPQRMRSRSR SFSRHRSCSR SPYSRSRSRS PGSRSSSRSC YYYESSHYRH RTHRNSPLYV RSRSRSPYSR RPRYDSYEEY QHERLKREEY RREYEKRESE RAKQRERQRQ KAIEERRVIY VGKIRPDTTR TELRDRFEVF GEIEECTVNL RDDGDSYGFI TYRYTCDAFA ALENGYTLRR SNETDFELYF CGRKQFFKSN YADLDSNSDD FDPASTKSKY DSLDFDSLLK EAQRSLRR
    • Molecular weight

      38 kDa
    • Amino acids

      481 to 798
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab124541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

  • Purity

    > 80 % .

  • Form

    Liquid
  • Additional notes

    Product was previously marketed under the MitoSciences sub-brand.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 78% PBS, 20% Glycerol, 1.75% Sodium chloride

General Info

  • Alternative names

    • L PGC 1alpha
    • LEM6
    • Ligand effect modulator 6
    • Peroxisome proliferative activated receptor gamma coactivator 1
    • Peroxisome proliferative activated receptor gamma coactivator 1 alpha
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT
    • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
    • PGC 1 (alpha)
    • PGC 1 alpha
    • PGC 1v
    • PGC-1-alpha
    • PGC1
    • PGC1(alpha)
    • PGC1A
    • PGC1v
    • PPAR gamma coactivator 1
    • PPAR gamma coactivator 1 alpha
    • PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6
    • PPAR gamma coactivator variant form
    • PPAR-gamma coactivator 1-alpha
    • PPARGC 1 alpha
    • PPARGC-1-alpha
    • PPARGC1
    • PPARGC1A
    • PRGC1_HUMAN
    see all
  • Function

    Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
  • Tissue specificity

    Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
    Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
    Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body.
  • Information by UniProt

Images

  • Lane 1 : Marker
    Lane 2 : PGC1 alpha protein (His tag) (ab124541) at 1 µg

    Gel: 10-20% acrylamide separation gel

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

    Stained with Coomassie Blue.
  • All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Recombinant Human PGC1 alpha protein (ab124541) at 0.01 µg

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 5 minutes

References

This product has been referenced in:

  • Yuan Y  et al. Activation of peroxisome proliferator-activated receptor-? coactivator 1a ameliorates mitochondrial dysfunction and protects podocytes from aldosterone-induced injury. Kidney Int 82:771-89 (2012). Read more (PubMed: 22648295) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

The recombinant protein ab124541 has not been tested in a functional assay, however it is only a fragment of the PGC1 alpha protein and it corresponds to amino acids 481-798. The PPARg binding region corresponds to amino acids 293-339 (according to UniProt accession Q9UBK2) so I do not expect this fragment to interact with PPARg.

Read More

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