Product nameRecombinant Human PGC1 alpha protein
See all PGC1 alpha proteins and peptides
Protein lengthProtein fragment
Amino Acid Sequence
SequenceTGELRDSDFS NEQFSKLPMF INSGLAMDGL FDDSEDESDK LSYPWDGTQS YSLFNVSPSC SSFNSPCRDS VSPPKSLFSQ RPQRMRSRSR SFSRHRSCSR SPYSRSRSRS PGSRSSSRSC YYYESSHYRH RTHRNSPLYV RSRSRSPYSR RPRYDSYEEY QHERLKREEY RREYEKRESE RAKQRERQRQ KAIEERRVIY VGKIRPDTTR TELRDRFEVF GEIEECTVNL RDDGDSYGFI TYRYTCDAFA ALENGYTLRR SNETDFELYF CGRKQFFKSN YADLDSNSDD FDPASTKSKY DSLDFDSLLK EAQRSLRR
Molecular weight38 kDa
Amino acids481 to 798
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab124541 in the following tested applications.
Purity> 80 % .
Product was previously marketed under the MitoSciences sub-brand.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 78% PBS, 20% Glycerol, 1.75% Sodium chloride
- L PGC 1alpha
- Ligand effect modulator 6
FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
Tissue specificityHeart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
Cellular localizationCytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body.
- Information by UniProt
Lane 1 : Marker
Lane 2 : PGC1 alpha protein (His tag) (ab124541) at 1 µg
Gel: 10-20% acrylamide separation gel
Predicted band size : 38 kDa
Observed band size : 38 kDa
Stained with Coomassie Blue.
All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml
Lane 1 : Marker
Lane 2 :
Recombinant Human PGC1 alpha protein (ab124541) at 0.01 µg
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 5 minutes
This product has been referenced in:
- Yuan Y et al. Activation of peroxisome proliferator-activated receptor-? coactivator 1a ameliorates mitochondrial dysfunction and protects podocytes from aldosterone-induced injury. Kidney Int 82:771-89 (2012). Read more (PubMed: 22648295) »