Recombinant Human PGK1 protein (ab199590)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
Description
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Product name
Recombinant Human PGK1 protein
See all PGK1 proteins and peptides -
Purity
> 95 % SDS-PAGE.
Determined by SEC-HPLC and reducing SDS-PAGE. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNL PQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKV NEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKIT LPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRA KQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCA KWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNILDHHHHHH -
Predicted molecular weight
46 kDa including tags -
Amino acids
2 to 417 -
Tags
His tag C-Terminus -
Additional sequence information
This product is for the mature full length protein. The initiator methionine is not included.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab199590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
HPLC
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8
Constituent: 100% PBS
Supplied as a 0.2 µm filtered solution
General Info
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Alternative names
- Cell migration-inducing gene 10 protein
- Epididymis secretory sperm binding protein Li 68p
- HEL S 68p
see all -
Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab199590 has not yet been referenced specifically in any publications.