Recombinant human PGK1 protein (ab211320)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human PGK1 protein
See all PGK1 proteins and peptides -
Biological activity
Specific activity: > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.
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Purity
> 95 % SDS-PAGE.
ab211320 was purified using conventional chromatography techniques. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMSLSNKLTLDKLDVKGKRVVMRVDFNVPMK NNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEP VAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEG KGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVN LPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDK VNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKI TLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTR AKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCC AKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI -
Predicted molecular weight
47 kDa including tags -
Amino acids
1 to 417 -
Tags
His tag N-Terminus -
Additional sequence information
NP_000282.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab211320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
Functional Studies
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cell migration-inducing gene 10 protein
- Epididymis secretory sperm binding protein Li 68p
- HEL S 68p
see all -
Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab211320 has been referenced in 1 publication.
- Bilsland AE et al. A Novel Pyrazolopyrimidine Ligand of Human PGK1 and Stress Sensor DJ1 Modulates the Shelterin Complex and Telomere Length Regulation. Neoplasia 21:893-907 (2019). PubMed: 31401411