Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      QLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLL LFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVAN NQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQ CRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVC REFTEREQGEVRFSAVALCKAA
    • Molecular weight

      25 kDa
    • Amino acids

      2 to 223
    • Tags

      His tag N-Terminus
    • Additional sequence information

      NM_004181.

Specifications

Our Abpromise guarantee covers the use of ab198431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity: 7433 pmol/min/µg.

    Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and PGP9.5. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, and emission at 460 nm.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    > 90 % SDS-PAGE.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.0
    Preservative: 1.53% Imidazole
    Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.05% DTT, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Epididymis luminal protein 117
    • Epididymis secretory protein Li 53
    • HEL 117
    • HEL S 53
    • NDGOA
    • Neuron cytoplasmic protein 9.5
    • OTTHUMP00000218137
    • OTTHUMP00000218139
    • OTTHUMP00000218140
    • OTTHUMP00000218141
    • Park 5
    • PARK5
    • PGP 9.5
    • PGP9.5
    • PGP95
    • Protein gene product 9.5
    • Ubiquitin C terminal esterase L1
    • Ubiquitin C terminal hydrolase
    • Ubiquitin C terminal hydrolase L1
    • Ubiquitin carboxyl terminal esterase L1
    • Ubiquitin carboxyl terminal hydrolase isozyme L1
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1
    • Ubiquitin thioesterase L1
    • Ubiquitin thiolesterase
    • Ubiquitin thiolesterase L1
    • UCH-L1
    • UCHL1
    • UCHL1_HUMAN
    see all
  • Function

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity

    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease

    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt

Images

  • 10% SDS-PAGE Coomassie staining 

    Lane 1: 5 µg ab198431 

    Lane 2: Protein Marker

  • Specific activity of ab198431.

References

ab198431 has not yet been referenced specifically in any publications.

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