Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human PGP9.5 protein (Tagged)
See all PGP9.5 proteins and peptides
Purity> 90 % SDS-PAGE.
The final product was refolded using a unique “emperature shift inclusion body refolding technology and chromatographically purified.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALL LLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVA NNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEG QCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKV CREFTEREQGEVRFSAVALC
Predicted molecular weight24 kDa
Amino acids1 to 220
TagsHis-T7 tag N-Terminus
Additional sequence informationMature protein contructed with a T7-His-TEV cleavage site Tag (29 amino acids) fusion at the N-terminal.
Our Abpromise guarantee covers the use of ab185598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1. May be used for in vitro UCHL1 protein mediated neuronal cell differentiation regulation study with “ProFectin” based intracellular delivery of this protein.
2. May be used for UCHL1 protein – protein interaction assay.
3. As Enzymatic substrate for various proteases.
4. Potential diagnostic biomarker protein for various cancer diseases.
5. May be used for specific antibody production.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and
- Epididymis luminal protein 117
- Epididymis secretory protein Li 53
- HEL 117
FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Involvement in diseaseParkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset
Sequence similaritiesBelongs to the peptidase C12 family.
Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab185598 has not yet been referenced specifically in any publications.