The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Chromosome 1 Open Reading Frame 12
Egl 9 family hypoxia inducible factor 1
EGL 9 homolog of C. elegans 1
EGL nine (C.elegans) homolog 1
Egl nine homolog 1
Egl nine homolog 1 (C. elegans)
Egl nine like protein 1
HIF Prolyl Hydroxylase 2
HIF-prolyl hydroxylase 2
Hypoxia inducible factor prolyl hydroxylase 2
Hypoxia-inducible factor prolyl hydroxylase 2
Prolyl Hydroxylase Domain Containing Protein 2
Prolyl hydroxylase domain-containing protein 2
Rat Homolog of SM20
Zinc finger MYND domain containing protein 6
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
Involvement in disease
Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.