Description

  • Product name

    Recombinant Human PHD3 protein
  • Purity

    > 90 % SDS-PAGE.
    ab128449 is purified using conventional chromatography techniques (anion exchange and gel filtration)
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED
    • Predicted molecular weight

      30 kDa including tags
    • Amino acids

      1 to 239
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab128449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.08% DTT, 0.32% Tris HCl, 0.06% EDTA, 50% Glycerol, 1.74% Sodium chloride

General Info

  • Alternative names

    • Egl 9 family hypoxia inducible factor 3
    • Egl nine homolog 3
    • Egl nine homolog 3 (C. elegans)
    • Egl nine like protein 3 isoform
    • EGL9 homolog of C. elegans 3
    • EGLN3
    • EGLN3_HUMAN
    • Factor responsive smooth muscle protein
    • HIF Prolyl Hydroxylase 3
    • HIF-PH3
    • HIF-prolyl hydroxylase 3
    • HIFP4H3
    • HIFPH3
    • HPH-1
    • HPH-3
    • Hypoxia-inducible factor prolyl hydroxylase 3
    • P4H3
    • PHD3
    • Prolyl Hydroxylase Domain Containing Protein 3
    • Prolyl hydroxylase domain-containing protein 3
    • SM20
    see all
  • Function

    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.
  • Tissue specificity

    Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
  • Sequence similarities

    Contains 1 Fe2OG dioxygenase domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab128449 at approximately 29.8 kDa (3µg).

References

ab128449 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

The ab128449 protein has never been tested for its enzymatic capacity. I am sorry we do not have any functionality data other than SDS page and MS.

If you are interested in testing this protein I can provide you 100% Abreview testing discount. Please click to link for more information

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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