Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)
- Datasheet
- References
- Protocols
Description
-
Product name
Recombinant human PHGDH/Malate dehydrogenase protein
See all PHGDH/Malate dehydrogenase proteins and peptides -
Biological activity
Specific Activity: ≥80 pmol/min/µg
Assay Conditions: The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl2, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid, 1 mM NAD, and various amount of ab198455 in a final volume of 50 µL. Reactions were initiated with the addition of ab198455, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature. -
Purity
> 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEG LIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPN GNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGI LGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLC DFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQ SGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIA VQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSP KGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEA GLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNG AVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLV SDGETWHVMGISSLLPSLEAWKQHVTEAFQFHFHHHHHH -
Predicted molecular weight
58 kDa including tags -
Amino acids
1 to 533 -
Tags
His tag C-Terminus -
Additional sequence information
GenBank Accession No. NM_006623
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab198455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
-
Form
Liquid -
Additional notes
This product was previously labelled as PHGDH
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8
Preservative: 1.16% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- 3 PGDH
- 3-PGDH
- 3-phosphoglycerate dehydrogenase
see all -
Pathway
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3. -
Involvement in disease
Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. - Information by UniProt
Images
-
Functional Studies - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)Specific activity of ab198455.
-
SDS-PAGE - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)4-20% SDS-PAGE analysis of ab198455 with Coomassie staining.
Lane 1: 5.5 µg ab198455
Lane 2: Protein marker
References
ab198455 has not yet been referenced specifically in any publications.
