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    recombinant-human-phgdhmalate-dehydrogenase-protein-ab198455.pdf

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Signal Transduction Metabolism Amino Acids
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Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)
  • SDS-PAGE - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 80% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human PHGDH/Malate dehydrogenase protein
    See all PHGDH/Malate dehydrogenase proteins and peptides
  • Biological activity

    Specific Activity: ≥80 pmol/min/µg
    Assay Conditions: The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl2, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid, 1 mM NAD, and various amount of ab198455 in a final volume of 50 µL. Reactions were initiated with the addition of ab198455, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature.

  • Purity

    > 80 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    O43175
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEG LIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPN GNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGI LGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLC DFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQ SGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIA VQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSP KGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEA GLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNG AVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLV SDGETWHVMGISSLLPSLEAWKQHVTEAFQFHFHHHHHH
    • Predicted molecular weight

      58 kDa including tags
    • Amino acids

      1 to 533
    • Tags

      His tag C-Terminus
    • Additional sequence information

      GenBank Accession No. NM_006623

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-PHGDH/Malate dehydrogenase antibody (ab57030)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab198455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as PHGDH

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.16% Imidazole
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 3 PGDH
    • 3-PGDH
    • 3-phosphoglycerate dehydrogenase
    • 3PGDH
    • D-3-phosphoglycerate dehydrogenase
    • EC 1.1.1.95
    • Epididymis secretory protein Li 113
    • HEL S 113
    • NLS
    • NLS1
    • PDG
    • PGAD
    • PGD
    • PGDH
    • PGDH3
    • Phgdh
    • PHGDHD
    • Phosphoglycerate dehydrogenase
    • SERA
    • SERA_HUMAN
    see all
  • Pathway

    Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.
  • Involvement in disease

    Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.
  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Target information above from: UniProt accession O43175 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)
    Functional Studies - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)

    Specific activity of ab198455.

  • SDS-PAGE - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)
    SDS-PAGE - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)

    4-20% SDS-PAGE analysis of ab198455 with Coomassie staining.

    Lane 1: 5.5 µg ab198455
    Lane 2: Protein marker

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab198455? Please let us know so that we can cite the reference in this datasheet.

    ab198455 has been referenced in 1 publication.

    • Intlekofer AM  et al. L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH. Nat Chem Biol 13:494-500 (2017). PubMed: 28263965

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Please use the links above to contact us or submit feedback about this product.

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