Recombinant human PHKG2 protein (ab89857)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human PHKG2 protein -
Biological activity
The specific activity of ab89857 was determined to be 70 nmol/min/mg. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab89857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab204885 (ZIP Kinase peptide substrate) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- GSD9C
- PHK gamma LT
- PHK gamma T
see all -
Relevance
Mutations in PHKG2 along with PHKA2 and PHKB, all three different genes of phosphorylase kinase (Phk) subunits, can give rise to glycogen storage disease of the liver. The autosomal-recessive, liver-specific variant of Phk deficiency is caused by mutations in the gene encoding the testis/liver isoform of the catalytic gamma subunit, PHKG2. -
Cellular localization
Cytoplasmic
Images
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Functional Studies - Recombinant human PHKG2 protein (ab89857)The specific activity of PHKG2 (ab89857) was determined to be 78 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human PHKG2 protein (ab89857)SDS PAGE analysis of ab89857 -
SDS-PAGE - Recombinant human PHKG2 protein (ab89857)SDS PAGE analysis of ab89857 -
Functional Studies - Recombinant human PHKG2 protein (ab89857)Kinase Assay demonstrating specific activity of ab89857 -
SDS-PAGE - Recombinant human PHKG2 protein (ab89857)SDS-PAGE showing ab89857 at approximately 70kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab89857 has not yet been referenced specifically in any publications.
