Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Suitable for: WB, SDS-PAGE
Product nameRecombinant Human PHLPP2 protein
Purity> 95 % Densitometry.
ab125556 is >95% pure.
Expression systemEscherichia coli
Protein lengthProtein fragment
Predicted molecular weight56 kDa including tags
Amino acids766 to 1043
Our Abpromise guarantee covers the use of ab125556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.003% EDTA, 0.79% Tris HCl, 0.004% DTT, 0.002% PMSF, 0.31% Glutathione
- PH domain and leucine rich repeat protein phosphatase 2
- PH domain leucine rich repeat protein phosphatase like
FunctionProtein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
Tissue specificityIn colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
Sequence similaritiesContains 22 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain.
Cellular localizationCytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125556 has not yet been referenced specifically in any publications.