Recombinant Human sPLA2-X protein (His tag) (ab63847)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB
Description
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Product name
Recombinant Human sPLA2-X protein (His tag) -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRGSHHHHHHGMASHMGILELAGTVGCVGPRTPIAYMKYGCFCGLGGHGQ PRDAIDWCCHGHDCCYTRAEEAGCSPKTERYSWQCVNQSVLCGPAENKCQ ELLCKCDQEIANCLAQTEYNLKYLFYPQFLCEPDSPKCD -
Predicted molecular weight
16 kDa including tags -
Amino acids
43 to 165 -
Tags
His tag N-Terminus -
Additional sequence information
Mature chain without signal peptide, without propeptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab63847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
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Form
Lyophilized -
Additional notes
Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.This product was previously labelled as Phospholipase A2 X
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.121% Tris
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ReconstitutionAdd deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
General Info
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Alternative names
- Group 10 secretory phospholipase A2
- Group X secretory phospholipase A2
- GX sPLA2
see all -
Function
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. -
Tissue specificity
Found in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon. -
Sequence similarities
Belongs to the phospholipase A2 family. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab63847 has not yet been referenced specifically in any publications.