Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein -
Expression system
Wheat germ -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLF HGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL -
Predicted molecular weight
37 kDa including tags -
Amino acids
1192 to 1291 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab112339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
Western blot
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Form
Liquid -
Additional notes
Best use within three months from the date of receipt of this protein.Previously labelled as Phospholipase C gamma 1.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
see all -
Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)ab112339 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
Datasheets and documents
References
ab112339 has not yet been referenced specifically in any publications.
