Recombinant Human PI-16 protein (ab117229)

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SDS-PAGE - Recombinant Human PI-16 protein (ab117229)

    Key features and details

    • Expression system: HEK 293 cells
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: DDDDK tag C-Terminus
    • Suitable for: WB, ELISA

    Description

    • Product name

      Recombinant Human PI-16 protein

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      HEK 293 cells

    • Accession

      Q6UXB8

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        LTDEEKRLMV ELHNLYRAQV SPPASDMLHM RWDEELAAFA KAYARQCVWG HNKERGRRGE NLFAITDEGM DVPLAMEEWH HEREHYNLSA ATCSPGQMCG HYTQVVWAKT ERIGCGSHFC EKLQGVEETN IELLVCNYEP PGNVKGKRPY QEGTPCSQCP SGYHCKNSLC EPIGSPEDAQ DLPYLVTEAP SFRATEASDS RKMGTPSSLA TGIPAFLVTE VSGSLATKAL PAVETQAPTS LATKDPPSMA TEAPPCVTTE VPSILAAHSL PSLDEEPVTF PKSTHVPIPK SADKVTDKTK VPSRSPENSL DPKMSLTGAR ELLPHAQEEA EAEAELPPSS EVLASVFPAQ DKPGELQATL DHTGHTSSKS LPNFPNTSAT ANATGGRALA LQSSLPGAEG PDKPSVVSGL NSGPGAAADY KDDDDK

      • Predicted molecular weight

        46 kDa including tags

      • Amino acids

        28 to 442

      • Tags

        DDDDK tag C-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab117229 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

    • Form

      Lyophilized

    • Additional notes

      Previously labelled as Peptidase inhibitor 16. 

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      Constituents: 0.32% Tris HCl, 0.3% Sodium chloride

    • Reconstitution
      Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

    General Info

    • Alternative names

      • Cripi
      • CRISP 9
      • CRISP-9
      • CRISP9
      • Cysteine rich protease inhibitor
      • Cysteine rich secretory protein 9
      • Cysteine-rich secretory protein 9
      • Microseminoprotein beta binding protein
      • MSMBBP
      • Peptidase inhibitor 16
      • PI-16
      • PI16
      • PI16_HUMAN
      • Protease inhibitor 16
      • PSP94 binding protein
      • PSP94-binding protein
      • PSPBP
      see all

    • Function

      Putative serine protease inhibitor.

    • Tissue specificity

      Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera.

    • Sequence similarities

      Belongs to the CRISP family.

    • Post-translational
      modifications

      N-glycosylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PI-16 protein (ab117229)
      SDS-PAGE - Recombinant Human PI-16 protein (ab117229)

      12% SDS-PAGE of Human PI-16
      1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa
      2. Reduced and boiled sample, 2µg / lane
      3. Non-reduced and non-boiled sample, 2µg / lane

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab117229? Please let us know so that we can cite the reference in this datasheet.

    ab117229 has not yet been referenced specifically in any publications.

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