Recombinant human PI 3 Kinase catalytic subunit gamma/PI3K-gamma protein (ab157600)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PI 3 Kinase catalytic subunit gamma/PI3K-gamma protein
See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma proteins and peptides -
Biological activity
~4.5nmol/mg/min using phosphatidylinositol as the substrate (lipid kinase activity test). -
Purity
> 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRK CKSPETALLHVAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLY QKKGQWYEIYDKYQVVQTLDCLRYWKATHRSPGQIHLVQRHPPSEESQAF QRQLTALIGYDVTDVSNVHDDELEFTRRGLVTPRMAEVASRDPKLYAMHP WVTSKPLPEYLWKKIANNCIFIVIHRSTTSQTIKVSPDDTPGAILQSFFT KMAKKKSLMDIPESQSEQDFVLRVCGRDEYLVGETPIKNFQWVRHCLKNG EEIHVVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKDHES VFTVSLWDCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRR TSPKPFTEEVLWNVWLEFSIKIKDLPKGALLNLQIYCGKAPALSSKASAE SPSSESKGKVQLLYYVNLLLIDHRFLLRRGEYVLHMWQISGKGEDQGSFN ADKLTSATNPDKENSMSISILLDNYCHPIALPKHQPTPDPEGDRVRAEMP NQLRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKW GQ QEIVAKTYQLLARREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQ KLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLF WFLRSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIEMLQKVT LDIKSLSAEKYDVSSQVISQLKQKLENLQNSQLPESFRVPYDPGLKAGAL AIEKCKVMASKKKPLWLEFKCADPTALSNETIGIIFKHGDDLRQDMLILQ ILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTV GNTG AFKDEVLNHWLKEKSPTEEKFQAAVERFVYSCAGYCVATFVLGI GDRHNDNIMITETGNLFHIDFGHILGNYKSFLGINKERVPFVLTPDFLFV MGTSGKKTSPHFQKFQDICVKAYLALRHHTNLLIILFSMMLMTGMPQLTS KEDIEYIRDALTVGKNEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVLGI KQGEKHSA -
Predicted molecular weight
126 kDa -
Amino acids
1 to 1102 -
Tags
His tag N-Terminus
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Associated products
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Related Products
- Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4H9] (ab139307)
- Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [OTI4G10] (ab140307)
- Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab154598)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody [Y388] (ab32089)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-PI 3 Kinase catalytic subunit gamma/PI3K-gamma antibody (ab70912)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab157600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Previously labelled as PI 3 Kinase catalytic subunit gamma.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
pH: 7.50
Constituents: 0.005% Magnesium chloride, 0.24% HEPES, 50% Glycerol, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 1 phosphatidylinositol 3 kinase
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma
- 5-bisphosphate 3-kinase catalytic subunit gamma isoform
see all -
Function
3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production. -
Tissue specificity
Pancreas, skeletal muscle, liver and heart. -
Pathway
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157600 has not yet been referenced specifically in any publications.