Recombinant human PI 3 Kinase catalytic subunit gamma/PI3K-gamma protein (ab157600)

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Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human PI 3 Kinase catalytic subunit gamma/PI3K-gamma protein
    See all PI 3 Kinase catalytic subunit gamma/PI3K-gamma proteins and peptides

  • Biological activity

    ~4.5nmol/mg/min using phosphatidylinositol as the substrate (lipid kinase activity test).

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    P48736

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRK CKSPETALLHVAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLY QKKGQWYEIYDKYQVVQTLDCLRYWKATHRSPGQIHLVQRHPPSEESQAF QRQLTALIGYDVTDVSNVHDDELEFTRRGLVTPRMAEVASRDPKLYAMHP WVTSKPLPEYLWKKIANNCIFIVIHRSTTSQTIKVSPDDTPGAILQSFFT KMAKKKSLMDIPESQSEQDFVLRVCGRDEYLVGETPIKNFQWVRHCLKNG EEIHVVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKDHES VFTVSLWDCDRKFRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRR TSPKPFTEEVLWNVWLEFSIKIKDLPKGALLNLQIYCGKAPALSSKASAE SPSSESKGKVQLLYYVNLLLIDHRFLLRRGEYVLHMWQISGKGEDQGSFN ADKLTSATNPDKENSMSISILLDNYCHPIALPKHQPTPDPEGDRVRAEMP NQLRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKW GQ QEIVAKTYQLLARREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQ KLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLF WFLRSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIEMLQKVT LDIKSLSAEKYDVSSQVISQLKQKLENLQNSQLPESFRVPYDPGLKAGAL AIEKCKVMASKKKPLWLEFKCADPTALSNETIGIIFKHGDDLRQDMLILQ ILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTV GNTG AFKDEVLNHWLKEKSPTEEKFQAAVERFVYSCAGYCVATFVLGI GDRHNDNIMITETGNLFHIDFGHILGNYKSFLGINKERVPFVLTPDFLFV MGTSGKKTSPHFQKFQDICVKAYLALRHHTNLLIILFSMMLMTGMPQLTS KEDIEYIRDALTVGKNEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVLGI KQGEKHSA

    • Predicted molecular weight

      126 kDa

    • Amino acids

      1 to 1102

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab157600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    Previously labelled as PI 3 Kinase catalytic subunit gamma. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    pH: 7.50
    Constituents: 0.005% Magnesium chloride, 0.24% HEPES, 50% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 1 phosphatidylinositol 3 kinase
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma
    • 5-bisphosphate 3-kinase catalytic subunit gamma isoform
    • p110 gamma
    • p110gamma
    • p120 PI3K
    • p120-PI3K
    • Phosphatidylinositol 3 kinase catalytic 110 kD gamma
    • Phosphatidylinositol 3 kinase gamma, p110 gamma
    • Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform
    • Phosphatidylinositol-4
    • Phosphoinositide 3 kinase catalytic gamma polypeptide
    • Phosphoinositide 3 kinase gamma catalytic subunit
    • PI 3 Kinase catalytic subunit gamma
    • PI3 kinase p110 subunit gamma
    • PI3-kinase subunit gamma
    • PI3CG
    • PI3K
    • PI3K-gamma
    • PI3Kgamma
    • PIK3
    • Pik3cg
    • PK3CG_HUMAN
    • PtdIns-3-kinase subunit gamma
    • PtdIns-3-kinase subunit p110-gamma
    • Serine/threonine protein kinase PIK3CG
    see all

  • Function

    3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.

  • Tissue specificity

    Pancreas, skeletal muscle, liver and heart.

  • Pathway

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab157600? Please let us know so that we can cite the reference in this datasheet.

ab157600 has not yet been referenced specifically in any publications.

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