Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      28 kDa
    • Amino acids

      326 to 575
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab127231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

     This product was previously labelled as PI 3 Kinase Class 2A

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • Reconstitution
    Reconstitute with water to desired concentration.

General Info

  • Alternative names

    • C2 containing phosphatidylinositol kinase
    • C2 domain containing alpha polypeptide
    • Class 2 alpha polypeptide
    • CPK
    • Cpk m
    • DKFZp686L193
    • MGC142218
    • OTTHUMP00000231763
    • P3C2A_HUMAN
    • Phosphatidylinositol 3 kinase class 2 alpha
    • Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
    • Phosphoinositide 3 kinase class 2 alpha
    • Phosphoinositide 3 kinase, class 2, alpha polypeptide
    • Phosphoinositide 3-Kinase-C2-alpha
    • PI3-K-C2(ALPHA)
    • PI3-K-C2A
    • PI3K-C2-alpha
    • PI3K-C2alpha
    • PI3KC 2 alpha
    • PIK3C 2A
    • Pik3c2a
    • PtdIns-3-kinase C2 subunit alpha
    see all
  • Function

    Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. May play a role in clathrin-coated endocytic vesicle formation and EGF signaling cascade. May be involved in mitosis and UV-induced damage response. May be a downstream effector in insulin signaling cascade.
  • Tissue specificity

    Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 C2 domain.
    Contains 1 PI3K/PI4K domain.
    Contains 1 PX (phox homology) domain.
  • Post-translational
    modifications

    Phosphorylated upon insulin stimulation; which may lead to enzyme activation (By similarity). Phosphorylated on Ser-259 during mitosis and upon UV irradiation; which does not change enzymatic activity but leads to proteasomal degradation. Ser-259 phosphorylation may be mediated by CDK1 or JNK, depending on the physiological state of the cell.
  • Cellular localization

    Cell membrane. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Nucleus. Cytoplasm. According to PubMed:10766823 and PubMed:11239472, it is found in the cell membrane, the Golgi apparatus and in clathrin-coated vesicles. According to PubMed:17038310 it inserts preferentially into membranes containing PtdIns(4,5)P2. According to PubMed:11606566, it is nuclear and cytoplasmic. Associated with RNA-containing structures. According to PubMed:14563213, it is mainly cytoplasmic.
  • Information by UniProt

References

ab127231 has not yet been referenced specifically in any publications.

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