Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
Product nameRecombinant human PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha protein
See all PI 3 Kinase p110 alpha + PI 3 kinase p85 alpha proteins and peptides
Biological activityThe specific activity of PI 3 Kinase p110alpha + PI 3 kinase p85 alpha was determined to be 50 nm/min/mg as per activity assay protocol.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab91093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.0308% DTT, 25% Glycerol, 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
RelevancePhosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols (PIs) at their 3´ OH position generating lipid second messengers and thereby regulate numerous biological processes including cell growth, differentiation, survival, proliferation, migration and metabolism. On the basis of structural similarities and substrate specificity, the PI3K family can be subdivided into three classes termed I, II, and III. All human class I members are heterodimers consisting of a catalytic subunit (MW approx. 110 kDa) and a non-catalytic subunit (MW 50, 55, 85, or 101 kDa) and are known to phosphorylate phosphatidylinositol (PI), phosphatidylinositol-4-mono-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2) in vitro. The class I members can be further subdivided into class IA and IB PI3Ks. Class IA exists in three isoforms (p110alpha, p110beta and p110delta whereas the only class IB member is termed p110gamma. Class IA PI3Ks are activated by adaptor proteins such as Ras or BCAP, or tyrosine-kinase-associated receptors including antigen, co-stimulatory and cytokine receptors (e.g. CD19, CD28, Insulin receptor, EGFR, and PDGFR). p110gamma is activated by G-protein-coupled receptors (GPCRs). Effectors of class I PI3Ks are pleckstrin homology domain proteins such as Akt/PKB, BTK, TEC, ITK, BAM32, and small GTPases (e.g. Cdc42, Rac, or Ras). The action of PI3Ks is regulated by the phosphatidylinositol- 3,4,5-trisphosphate phosphatases SHIP and PTEN.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91093 has not yet been referenced specifically in any publications.