Recombinant Human PI 3 Kinase p85 alpha protein (ab123752)

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SDS-PAGE - Recombinant Human PI 3 Kinase p85 alpha protein (ab123752)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human PI 3 Kinase p85 alpha protein
      See all PI 3 Kinase p85 alpha proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      P27986

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Predicted molecular weight

        86 kDa including tags

      • Amino acids

        1 to 724

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab123752 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      Centrifuge the vial prior to opening.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.00
      Preservative: 1.02% Imidazole
      Constituents: 1.75% Sodium chloride, 25% Glycerol, 0.03% DTT, 0.82% Sodium phosphate, 0.002% PMSF

    General Info

    • Alternative names

      • GRB1
      • p85
      • p85 alpha
      • P85A_HUMAN
      • Phosphatidylinositol 3 kinase associated p 85 alpha
      • Phosphatidylinositol 3 kinase regulatory 1
      • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha)
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
      • Phosphatidylinositol 3-kinase regulatory subunit alpha
      • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha)
      • PI3 kinase p85
      • PI3 kinase p85 subunit alpha
      • PI3-kinase regulatory subunit alpha
      • PI3-kinase subunit p85-alpha
      • PI3K
      • PI3K p85
      • PI3K regulatory subunit alpha
      • Pik3r1
      • PtdIns 3 kinase p85 alpha
      • PtdIns-3-kinase regulatory subunit alpha
      • PtdIns-3-kinase regulatory subunit p85-alpha
      see all

    • Function

      Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

    • Tissue specificity

      Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

    • Sequence similarities

      Belongs to the PI3K p85 subunit family.
      Contains 1 Rho-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.

    • Domain

      The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.

    • Post-translational
      modifications

      Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
      Phosphorylated. Dephosphorylated by PTPRJ.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PI 3 Kinase p85 alpha protein (ab123752)
      SDS-PAGE - Recombinant Human PI 3 Kinase p85 alpha protein (ab123752)
      SDS-PAGE showing ab123752 at approximately 86kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab123752? Please let us know so that we can cite the reference in this datasheet.

    ab123752 has been referenced in 1 publication.

    • Zhou K  et al. MicroRNA-145 inhibits the activation of the mTOR signaling pathway to suppress the proliferation and invasion of invasive pituitary adenoma cells by targeting AKT3 in vivo and in vitro. Onco Targets Ther 10:1625-1635 (2017). PubMed: 28352194

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