Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human PI4KA protein (Active)
The specific activity of ab268862 was 70 nmol/min/mg in a kinase assay using PI:PS as substrate.
Purity> 70 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Molecular weight information120 kDa by SDS-PAGE
Amino acids1 to 2102
TagsGST tag N-Terminus
Additional sequence informationGenbank: NM_002650
Our Abpromise guarantee covers the use of ab268862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 126.96.36.199
- Phosphatidylinositol 4 kinase alpha
- Phosphatidylinositol 4 kinase catalytic alpha polypeptide
FunctionActs on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
Tissue specificityExpressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes.
Sequence similaritiesBelongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
Contains 1 PI3K/PI4K domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268862 has not yet been referenced specifically in any publications.