Product nameRecombinant Human PIAS1 protein
See all PIAS1 proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
SequenceMADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCS PAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYD GHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDEL IKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQL RFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPI NITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAK GIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALT CSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCT DCDEIQFKEDGTWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQS SNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPH QASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLS GDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTS LPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISL D
Molecular weight98 kDa including tags
Amino acids1 to 651
Our Abpromise guarantee covers the use of ab152888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein concentration is above or equal to 0.05 mg/ml. Best use within three months from the date of receipt of this protein.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.79% Tris HCl
- AR interacting protein
- DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
Tissue specificityExpressed in numerous tissues with highest level in testis.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
DomainThe LXXLL motif is a transcriptional coregulator signature.
The SP-RING-type domain is required for promoting EKLF sumoylation.
Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
Cellular localizationNucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
- Information by UniProt
Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution
All lanes :
Recombinant Human PIAS1 protein (ab152888)
Lysates/proteins at 0.01 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 98 kDa why is the actual band size different from the predicted?
Lane 1: 10 seconds.
Lane 2: 1 minute.
Blocking and dilution buffer: 5% NFDM/TBST.
12.5% SDS-PAGE analysis of ab152888 stained with Coomassie Blue.
ab152888 has not yet been referenced specifically in any publications.