Overview

  • Product name

    Recombinant Human PIAS3 protein
  • Protein length

    Protein fragment

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      27 kDa
    • Amino acids

      274 to 521

Specifications

Our Abpromise guarantee covers the use of ab125996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • Reconstitution
    Reconstitute with water to desired concentration.

General Info

  • Alternative names

    • E3 SUMO protein ligase PIAS 3
    • E3 SUMO protein ligase PIAS3
    • E3 SUMO-protein ligase PIAS3
    • FLJ14651
    • OTTHUMP00000015586
    • OTTHUMP00000015587
    • PIAS 3
    • Pias3
    • PIAS3 protein
    • PIAS3_HUMAN
    • Protein inhibitor of activated STAT 3
    • Protein inhibitor of activated STAT protein 3
    • Protein inhibitor of activated STAT3
    • Zinc finger MIZ type containing 5
    • ZMIZ 5
    • ZMIZ5
    see all
  • Function

    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
  • Tissue specificity

    Widely expressed.
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • Domain

    The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
    The LXXLL motif is a transcriptional coregulator signature.
  • Post-translational
    modifications

    Sumoylated.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
  • Information by UniProt

References

ab125996 has not yet been referenced specifically in any publications.

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