Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
Product nameRecombinant Human PICH protein
Expression systemWheat germ
Protein lengthFull length protein
SequenceMEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNL DQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDA QASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSL CGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGE DEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPS SVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGV EESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLS GEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKS ADPEVMLLTLSLYKQLNNN
Amino acids1 to 419
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab162850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as ERCC6L.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.79% Tris HCl
- ATP dependent helicase ERCC6 like
- ATP-dependent helicase ERCC6-like
- DNA excision repair protein ERCC 6 like
FunctionDNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 TPR repeats.
modificationsPhosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
Cellular localizationChromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab162850 has not yet been referenced specifically in any publications.