Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human Pik3ca + PIK3R1 protein
Biological activityThe Specific activity of ab85833 was determined to be 5147 RLU/min/mg.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab85833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.0308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
RelevanceThe enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab85833 has not yet been referenced specifically in any publications.