Recombinant human PIM2 protein (ab60836)
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- References
- Protocols
Description
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Product name
Recombinant human PIM2 protein
See all PIM2 proteins and peptides -
Purity
> 95 % SDS-PAGE.
Purity: >95% as determined by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
GST tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- OTTHUMP00000025825
- Pim 2 oncogene
- Pim 2h
see all -
Function
Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate. -
Tissue specificity
Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. - Information by UniProt
Images
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Functional Studies - Recombinant human PIM2 protein (ab60836)The specific activity of PIM2 (ab60836) was determined to be 270 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human PIM2 protein (ab60836)SDS PAGE analysis of ab60836 -
Functional Studies - Recombinant human PIM2 protein (ab60836)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PIM2 protein (ab60836)ab60836 on SDS-PAGE, MW ~61kDa.
Datasheets and documents
References
ab60836 has not yet been referenced specifically in any publications.
