Recombinant Human PIMT protein (Tagged) (ab239540)

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Description

  • Product name

    Recombinant Human PIMT protein (Tagged)

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    Q96RS0

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFL SPPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADID QVASLAGPGGQVEIEQNFLNNKLKTITAYFGDLIRRPASET

    • Predicted molecular weight

      32 kDa including tags

    • Amino acids

      713 to 853

    • Tags

      His tag N-Terminus

    • Additional sequence information

      N-terminal 6xHis-SUMO-tag.

Specifications

Our Abpromise guarantee covers the use of ab239540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituents: Tris buffer, 50% Glycerol

General Info

  • Alternative names

    • Cap specific guanine N2 methyltransferase
    • Cap-specific guanine-N2 methyltransferase
    • CLL associated antigen KW 2
    • CLL-associated antigen KW-2
    • DKFZp762A163
    • FLJ22995
    • HCA137
    • Hepatocellular carcinoma associated antigen 137
    • Hepatocellular carcinoma-associated antigen 137
    • NCOA6IP
    • Nuclear receptor coactivator 6 interacting protein
    • Nuclear receptor coactivator 6-interacting protein
    • PIMT
    • PIPMT
    • PRIP interacting protein PIPMT
    • PRIP interacting protein with methyltransferase domain
    • PRIP interacting protein with methyltransferase motif
    • PRIP-interacting protein with methyltransferase motif
    • SEREX defined
    • TGS 1
    • Tgs1
    • TGS1_HUMAN
    • Trimethylguanosine synthase
    • Trimethylguanosine synthase homolog
    • Trimethylguanosine synthase homolog (S. cerevisiae)
    see all

  • Function

    Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.

  • Tissue specificity

    Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.

  • Sequence similarities

    Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.

  • Cellular localization

    Cytoplasm. Nucleus > Cajal body. A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PIMT protein (Tagged) (ab239540)
    SDS-PAGE - Recombinant Human PIMT protein (Tagged) (ab239540)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab239540.

References

ab239540 has not yet been referenced specifically in any publications.

Publishing research using ab239540? Please let us know so that we can cite the reference in this datasheet.

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