Recombinant Human PIMT protein (Tagged) (ab239540)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human PIMT protein (Tagged) -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFL SPPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADID QVASLAGPGGQVEIEQNFLNNKLKTITAYFGDLIRRPASET -
Predicted molecular weight
32 kDa including tags -
Amino acids
713 to 853 -
Tags
His tag N-Terminus -
Additional sequence information
N-terminal 6xHis-SUMO-tag.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab239540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Cap specific guanine N2 methyltransferase
- Cap-specific guanine-N2 methyltransferase
- CLL associated antigen KW 2
see all -
Function
Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation. -
Tissue specificity
Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta. -
Sequence similarities
Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family. -
Cellular localization
Cytoplasm. Nucleus > Cajal body. A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PIMT protein (Tagged) (ab239540)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab239540.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab239540 has not yet been referenced specifically in any publications.
