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I have a few questions about your product ab116177 which is the recombinant protein PINK1.
How was the protein purified?
Does it have a tag?
Why is 1M urea in the final buffer?
How do you know it is autophosphoryated?
Is it active?
E. Wheatley PhD
Cancer Research Technology Ltd
Wolfson Institute for Biomedical Research
London WC1E 6BT
Tel: (+44) 020 76790927
Fax:(+44) 020 7796 3907
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Asked on Jul 17 2012
Thank you for your enquiry.
I am pleased to help provide some answers:
Q1. How was the protein purified? I am sorry to confirm that this particular product is sourced externally and we regrettably are not able to share manufacturing process information on this occasion.
Q2. Does it have a tag? No, it does not have a tag.
Q3. Why is 1 M urea in the final buffer? The urea helps prevent aggregation.
Q4. Do you know if the protein is phosphorylated? If so, how has this been assessed? We have not confirmed if this protein is phosphorylated, but we suspect it isn’t. It’s most likely denatured.
Q5. Is the protein active? I am sorry this has not been tested. It is unlikely to be active if it is denatured.
I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.
Answered on Jul 17 2012