Recombinant human PIP5K3/PIKFYVE protein (ab207977)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human PIP5K3/PIKFYVE protein
See all PIP5K3/PIKFYVE proteins and peptides -
Biological activity
The specific activity of ab207977 was determined to be 110 nmol/min/mg
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Purity
> 70 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
110 kDa including tags -
Amino acids
1400 to 2098 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_015040
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Specifications
Our Abpromise guarantee covers the use of ab207977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
This product was previously labelled as PIP5K3
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 1 phosphatidylinositol 3 phosphate 5 kinase
- 1 phosphatidylinositol 4 phosphate 5 kinase
- 1-phosphatidylinositol 3-phosphate 5-kinase
see all -
Function
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes. -
Involvement in disease
Corneal dystrophy, fleck -
Sequence similarities
Contains 1 DEP domain.
Contains 1 FYVE-type zinc finger.
Contains 1 PIPK domain. -
Cellular localization
Endosome membrane. Mainly associated with membranes of the late endocytic pathway. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab207977 has not yet been referenced specifically in any publications.