Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human PIP5K3/PIKFYVE protein
See all PIP5K3/PIKFYVE proteins and peptides
The specific activity of ab207977 was determined to be 110 nmol/min/mg
Purity> 70 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight110 kDa including tags
Amino acids1400 to 2098
TagsGST tag N-Terminus
Additional sequence informationNM_015040
Our Abpromise guarantee covers the use of ab207977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as PIP5K3
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 1 phosphatidylinositol 3 phosphate 5 kinase
- 1 phosphatidylinositol 4 phosphate 5 kinase
- 1-phosphatidylinositol 3-phosphate 5-kinase
FunctionThe PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.
Involvement in diseaseCorneal dystrophy, fleck
Sequence similaritiesContains 1 DEP domain.
Contains 1 FYVE-type zinc finger.
Contains 1 PIPK domain.
Cellular localizationEndosome membrane. Mainly associated with membranes of the late endocytic pathway.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab207977 has not yet been referenced specifically in any publications.