Recombinant human PKA beta (catalytic subunit) protein (ab60837)

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Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
  • Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

Description

  • Product name

    Recombinant human PKA beta (catalytic subunit) protein
    See all PKA beta (catalytic subunit) proteins and peptides

  • Purity

    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      65 kDa including tags

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
    • cAMP-dependent protein kinase catalytic subunit beta
    • KAPCB_HUMAN
    • PKA C beta
    • PKA C-beta
    • PKACB
    • Prkacb
    • protein kinase A catalytic subunit beta
    • Protein kinase cAMP dependent catalytic beta
    see all

  • Function

    Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

  • Tissue specificity

    Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.

  • Cellular localization

    Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    The specific activity of PKA beta (catalytic subunit) (ab60837) was determined to be 430 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS PAGE analysis of ab60837
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS PAGE analysis of ab60837
  • Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    ab60837 on SDS-PAGE, MW ~65 kDa.
  • Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab60837? Please let us know so that we can cite the reference in this datasheet.

ab60837 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

PKA beta (catalytic subunit) protein (Active) (ab60837)

I have not been able to get the enzyme to phosphorylate a known substrate that I have made, i have tried several conditions as theproduct information sheet doesn't specify the best assay conditions. I am not sure whether the problem is with the enzyme or the substrate - do you sell/can you recommend a substrate that I can use as a control for PKA?

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I contacted the source of this protein and they would liketo receivedetailed information with regards to your protocol, substrate information, lot number.
They need to have a thorough understanding of whathas beenperformed prior to make recommendations
This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the protein.

I look forward to receiving your reply.

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Question

Hi
I have recently purchased your an abcam product through sapphire biosciences


PKA beta (catalytic subunit) protein (Active) (ab60837)


I have not been able to get the enzyme to phosphorylate a known substrate that I have made, i have tried several conditions as theproduct information sheet doesn't specify the best assay conditions. I am not sure whether the problem is with the enzyme or the substrate - do you sell/can you recommend a substrate that I can use as a control for PKA?

Thanks
--

Read More
Answer

Thank you for your inquiry.

I can confirm that ab60837 was used in a kinase assay with CREBtide synthetic peptide substrate (KRREILSRRPSYR) , stock 1mg/ml.

This is available for purchase from other sources.

I attached the kinase assay protocol for your information.

Please let me know if the problems persist with the positive control.

We are happy to confirm that this product is tested and guaranteed for activity assays. In the event that a product is not functioning as stated on the datasheet, we happily offer technical help and a free of charge replacement or credit note.

https://www.abcam.com/index.html?pageconfig=resource&rid=10372&pid=10000&source=pagetrap&viapagetrap=abpromise

I wish you good luck with your research.

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