Recombinant human PKA beta (catalytic subunit) protein (ab60837)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      65 kDa including tags
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Other

    Western blot

  • Purity
    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Form
    Liquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
    • cAMP-dependent protein kinase catalytic subunit beta
    • KAPCB_HUMAN
    • PKA C beta
    • PKA C-beta
    • PKACB
    • Prkacb
    • protein kinase A catalytic subunit beta
    • Protein kinase cAMP dependent catalytic beta
    see all
  • Function
    Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
  • Tissue specificity
    Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    SDS-PAGE - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    ab60837 on SDS-PAGE, MW ~65 kDa.
  • Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)
    Western blot - Recombinant human PKA beta (catalytic subunit) protein (ab60837)

References

ab60837 has not yet been referenced specifically in any publications.

Publishing research using ab60837? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
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PKA beta (catalytic subunit) protein (Active) (ab60837)

I have not been able to get the enzyme to phosphorylate a known substrate that I have made, i have tried several conditions as theproduct information sheet doesn't specify the best assay ...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I conta...

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Hi
I have recently purchased your an abcam product through sapphire biosciences


PKA beta (catalytic subunit) protein (Active) (ab60837)


I have not been able to get the enzyme to phosphorylate a known substrate tha...

Read More

Thank you for your inquiry.

I can confirm that ab60837 was used in a kinase assay with CREBtide synthetic peptide substrate (KRREILSRRPSYR) , stock 1mg/ml.

This is available for purchase from other sources.

I attached the ...

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