Description

  • Product name

    Recombinant human PKC epsilon protein
    See all PKC epsilon proteins and peptides
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 1.74% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • KPCE_HUMAN
    • MGC125656
    • MGC125657
    • nPKC epsilon
    • nPKC-epsilon
    • PKCE
    • Pkcea
    • PRKCE
    • Protein kinase C epsilon
    • Protein kinase C epsilon type
    see all
  • Function

    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt

Images

  • The specific activity of PKC epsilon (ab60847) was determined to be 1122 nmol/min/mg as per activity assay protocol and was equivalent to 1398 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab60847
  • SDS PAGE analysis of ab60847
  • Sample Kinase Activity Plot.
  • ab60847 on SDS-PAGE, MW ~110 kDa.

References

ab60847 has not yet been referenced specifically in any publications.

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