Product nameRecombinant human PKC epsilon protein
See all PKC epsilon proteins and peptides
Purity> 90 % SDS-PAGE.
ab73538 is purified by proprietary chromatographic techniques and filter sterilized.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Amino acids2 to 737
Our Abpromise guarantee covers the use of ab73538 in the following tested applications.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.03% Brij, 0.012% Benzamidine, 0.00348% PMSF, 0.0154% DTT, 0.242% Tris, 0.00292% EDTA, 20% Glycerol, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
modificationsPhosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab73538 has not yet been referenced specifically in any publications.