Description

  • Product name

    Recombinant human PKC gamma protein
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • KPCG_HUMAN
    • MGC57564
    • OTTHUMP00000067291
    • PKC-gamma
    • PKCC
    • PKCG
    • PRKCG
    • Protein kinase C gamma
    • Protein kinase C gamma polypeptide
    • Protein kinase C gamma type
    • Protein kinase C, gamma
    • SCA 14
    • SCA14
    see all
  • Function

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificity

    Expressed in Purkinje cells of the cerebellar cortex.
  • Involvement in disease

    Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Information by UniProt

Images

  • The specific activity of PKC gamma (ab60842) was determined to be 586 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab60842
  • SDS PAGE analysis of ab60842
  • Sample Kinase Activity Plot.
  • ab60842 on SDS-PAGE, MW ~105 kDa.

References

ab60842 has not yet been referenced specifically in any publications.

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