Recombinant human PKC mu/PKD protein (ab60873)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
Description
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Product name
Recombinant human PKC mu/PKD protein -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Western blot
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKC mu
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- KPCD1_HUMAN
- nPKC D1
- nPKC mu
see all -
Function
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
Images
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Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)The specific activity of PKC mu/PKD (ab60873) was determined to be 650 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)SDS PAGE analysis of ab60873 -
SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)SDS PAGE analysis of ab60873 -
Western blot - Recombinant human PKC mu/PKD protein (ab60873)Ab5283 recognizes the full length tagged recombinant PKC mu protein (ab60873) which has an expected molecular weight of 131 kDa. -
Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)ab60873 on SDS-PAGE, MW ~131 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab60873 has not yet been referenced specifically in any publications.
