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  1. Link

    recombinant-human-pkc-mupkd-protein-ab60873.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Recombinant human PKC mu/PKD protein (ab60873)

  • Datasheet
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Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)
  • SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
  • SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
  • Western blot - Recombinant human PKC mu/PKD protein (ab60873)
  • Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)
  • SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies, WB

Description

  • Product name

    Recombinant human PKC mu/PKD protein
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

  • Related Products

    • Anti-PKC mu/PKD antibody [EP1493Y] (ab51246)
    • Anti-PKC mu/PKD (phospho S205) antibody (ab59413)
    • Anti-PKC mu/PKD (phospho Y463) antibody (ab59415)
  • Substrate reagent

    • CREB peptide (ab204856)

Specifications

Our Abpromise guarantee covers the use of ab60873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Form

    Liquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

     This product was previously labelled as PKC mu

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • KPCD1_HUMAN
    • nPKC D1
    • nPKC mu
    • nPKC-D1
    • nPKC-mu
    • nPKCD1
    • nPKCmu
    • PKC
    • PKC MU
    • PKCM
    • PKCmu
    • PKD
    • PKD 1
    • PKD1
    • PRKCM
    • PRKD 1
    • Prkd1
    • Protein kinase C mu
    • Protein kinase C mu type
    • Protein kinase D
    • Protein kinase D1
    • Serine/threonine protein kinase D1
    • Serine/threonine-protein kinase D1
    see all
  • Function

    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Target information above from: UniProt accession Q15139 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)
    Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)
    The specific activity of PKC mu/PKD (ab60873) was determined to be 650 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
    SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
    SDS PAGE analysis of ab60873
  • SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
    SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
    SDS PAGE analysis of ab60873
  • Western blot - Recombinant human PKC mu/PKD protein (ab60873)
    Western blot - Recombinant human PKC mu/PKD protein (ab60873)
    Ab5283 recognizes the full length tagged recombinant PKC mu protein (ab60873) which has an expected molecular weight of 131 kDa.
  • Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)
    Functional Studies - Recombinant human PKC mu/PKD protein (ab60873)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
    SDS-PAGE - Recombinant human PKC mu/PKD protein (ab60873)
    ab60873 on SDS-PAGE, MW ~131 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab60873? Please let us know so that we can cite the reference in this datasheet.

    ab60873 has not yet been referenced specifically in any publications.

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