Recombinant human PKC nu/PRKD3 protein (ab60874)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant human PKC nu/PRKD3 protein -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKC nu
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EPK 2
- EPK2
- KPCD3_HUMAN
see all -
Function
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
Images
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Functional Studies - Recombinant human PKC nu/PRKD3 protein (ab60874)The specific activity of PKC nu/PRKD3 (ab60874) was determined to be 108 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human PKC nu/PRKD3 protein (ab60874)SDS PAGE analysis of ab60874 -
SDS-PAGE - Recombinant human PKC nu/PRKD3 protein (ab60874)SDS PAGE analysis of ab60874 -
Functional Studies - Recombinant human PKC nu/PRKD3 protein (ab60874)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC nu/PRKD3 protein (ab60874)ab60874 on SDS-PAGE, MW ~142 kDa.
Datasheets and documents
References
ab60874 has not yet been referenced specifically in any publications.
