Recombinant human PKC nu/PRKD3 protein (ab60874)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human PKC nu/PRKD3 protein -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity.
This product was previously labelled as PKC nu.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EPK 2
- EPK2
- KPCD3_HUMAN
see all -
Function
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
Images
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Functional Studies - Recombinant human PKC nu/PRKD3 protein (ab60874)The specific activity of PKC nu/PRKD3 (ab60874) was determined to be 108 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PKC nu/PRKD3 protein (ab60874)SDS PAGE analysis of ab60874 -
SDS-PAGE - Recombinant human PKC nu/PRKD3 protein (ab60874)SDS PAGE analysis of ab60874 -
Functional Studies - Recombinant human PKC nu/PRKD3 protein (ab60874)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC nu/PRKD3 protein (ab60874)ab60874 on SDS-PAGE, MW ~142 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab60874 has not yet been referenced specifically in any publications.
