Description

  • Product name

    Recombinant human PKC zeta protein
  • Purity

    > 90 % SDS-PAGE.
    Purity: >90% as determined by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab60848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 14-3-3-zetaisoform
    • AI098070
    • aPKCzeta
    • C80388
    • EC 2.7.11.13
    • KPCZ_HUMAN
    • nPKC zeta
    • nPKC-zeta
    • OTTHUMP00000001368
    • OTTHUMP00000044160
    • PKC 2
    • PKC ZETA
    • PKC2
    • Pkcz
    • PKCZETA
    • PKM-zeta, included
    • PRKCZ
    • Protein kinase C zeta
    • Protein kinase C zeta form
    • Protein kinase C zeta type
    • r14-3-3
    • R74924
    • zetaPKC
    see all
  • Function

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • Tissue specificity

    Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • Domain

    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • Post-translational
    modifications

    CDH5 is required for its phosphorylation at Thr-410.
  • Cellular localization

    Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt

Images

  • The specific activity of PKC zeta (ab60848) was determined to be 103 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab60848
  • SDS PAGE analysis of ab60848
  • Sample Kinase Activity Plot.
  • ab60848 on SDS-PAGE, MW ~93 kDa.

References

ab60848 has not yet been referenced specifically in any publications.

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