Recombinant human PKC zeta protein (ab60848)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
-
Product name
Recombinant human PKC zeta protein -
Purity
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Tags
GST tag N-Terminus
-
Associated products
-
Related Products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
SDS-PAGE
Western blot
-
Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- 14-3-3-zetaisoform
- AI098070
- aPKCzeta
see all -
Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
Tissue specificity
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Domain
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsCDH5 is required for its phosphorylation at Thr-410. -
Cellular localization
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
Images
-
Functional Studies - Recombinant human PKC zeta protein (ab60848)The specific activity of PKC zeta (ab60848) was determined to be 103 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant human PKC zeta protein (ab60848)SDS PAGE analysis of ab60848 -
SDS-PAGE - Recombinant human PKC zeta protein (ab60848)SDS PAGE analysis of ab60848 -
Functional Studies - Recombinant human PKC zeta protein (ab60848)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC zeta protein (ab60848)ab60848 on SDS-PAGE, MW ~93 kDa. -
Western blot - Recombinant human PKC zeta protein (ab60848)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab60848 has not yet been referenced specifically in any publications.
