Description

  • Product name

    Recombinant human PKLR protein
    See all PKLR proteins and peptides
  • Biological activity

    Specific Activity: ≥38 pmol/min/µg.

     

    A 50 µl reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 10 mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected.

  • Purity

    >= 93 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      EGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEP VAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIA NVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQ VLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIG PEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDI VFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDG IMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPR PTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAV YHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQ LLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRR VQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS
    • Predicted molecular weight

      59 kDa including tags
    • Amino acids

      2 to 543
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Isoform L-type. NM_181871.

Specifications

Our Abpromise guarantee covers the use of ab196117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.63% Tris HCl, 0.85% Sodium chloride, 20% Glycerol, 0.05% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • EC 2.7.1.40
    • KPYR_HUMAN
    • L-PK
    • Pk-1
    • PK1
    • PKL
    • Pklg
    • Pklr
    • PKR
    • PKRL
    • Pyruvate kinase 1
    • Pyruvate kinase isozymes R/L
    • Pyruvate kinase liver and blood cell
    • Pyruvate kinase liver and RBC
    • Pyruvate kinase liver and red blood cell
    • Pyruvate kinase liver type
    • Pyruvate kinase type L
    • Pyruvate kinase, red cell type
    • R type/L type pyruvate kinase
    • R-PK
    • R-type/L-type pyruvate kinase
    • Red cell/liver pyruvate kinase
    • RPK
    see all
  • Function

    Plays a key role in glycolysis.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • Involvement in disease

    Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
    Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
  • Sequence similarities

    Belongs to the pyruvate kinase family.
  • Information by UniProt

Images

  • 10% SDS-PAGE analysis of 8 µg ab196117 with Coomassie staining.

  • Kinase assay using ab196117 showing the specific activity as ≥38 pmol/min/µg.

References

ab196117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196117.
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