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Bioactive grade

Recombinant human PKM2 protein (Active) (ab89364)

Reviews (1) Submit a question References (2)
SDS-PAGE - Recombinant human PKM2 protein (ab89364)
  • Western blot - Recombinant human PKM2 protein (ab89364)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: Functional Studies, WB, SDS-PAGE

Description

  • Product name

    Recombinant human PKM2 protein (Active)
    See all PKM2 proteins and peptides

  • Biological activity

    Specific activity: > 25,000 pmol/min/ug.

    One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

  • Purity

    > 95 % SDS-PAGE.
    ab89364 is purified using conventional chromatography techniques.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P14618-1

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P

    • Predicted molecular weight

      60 kDa

    • Amino acids

      1 to 531

    • Tags

      His tag N-Terminus

    • Additional sequence information

      (NP_002645)

Specifications

Our Abpromise guarantee covers the use of ab89364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    Endotioxin Levels determined by LAL method.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CTHBP
    • Cytosolic thyroid hormone binding protein
    • Cytosolic thyroid hormone-binding protein
    • KPYM_HUMAN
    • MGC3932
    • OIP 3
    • OIP-3
    • OIP3
    • OPA interacting protein 3
    • Opa-interacting protein 3
    • p58
    • PK muscle type
    • PK, muscle type
    • PK2
    • PK3
    • PKM
    • PKM2
    • pykm
    • Pyruvate kinase 2/3
    • Pyruvate kinase 3
    • Pyruvate kinase isozymes M1/M2
    • Pyruvate kinase muscle
    • Pyruvate kinase muscle isozyme
    • pyruvate kinase PKM
    • Pyruvate kinase, muscle 2
    • TCB
    • THBP1
    • Thyroid hormone binding protein 1
    • Thyroid hormone binding protein cytosolic
    • Thyroid hormone-binding protein 1
    • Tumor M2 PK
    • Tumor M2-PK
    see all

  • Function

    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

  • Tissue specificity

    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

  • Sequence similarities

    Belongs to the pyruvate kinase family.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.

  • Cellular localization

    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human PKM2 protein (ab89364)
    SDS-PAGE - Recombinant human PKM2 protein (ab89364)

    3ug of reduced ab89364 on SDS-PAGE, stained with Coomassie Blue.

  • Western blot - Recombinant human PKM2 protein (ab89364)
    Western blot - Recombinant human PKM2 protein (ab89364)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab89364? Please let us know so that we can cite the reference in this datasheet.

ab89364 has been referenced in 2 publications.

  • Zhang W  et al. FOXM1D potentiates PKM2-mediated tumor glycolysis and angiogenesis. Mol Oncol 15:1466-1485 (2021). PubMed: 33314660
  • Xiao M  et al. The eEF2 kinase-induced STAT3 inactivation inhibits lung cancer cell proliferation by phosphorylation of PKM2. Cell Commun Signal 18:25 (2020). PubMed: 32054489

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Other (in vitro activity assay) Abreview for PKM2 protein (Active)

 Excellent
Abreviews
Application
Other - Do not use

Review text: The protein is of a excellent quality and it is working for functional assays.
Sample: Human Purified protein

Primary antibody (in addition to 'PKM2 protein (Active)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
Abcam response: We would like to thank you for taking thing time to submit an Abreview for this product. With publication of this review you have gained Abpoints which may be redeemed for future products or gift cards! If you submit an image which we publish with the Abreview, you will get an additional 100 Abpoints!

If you don't have an image at the moment, then submit your Abreview now, and email us your image later to receive your extra 100 Abpoints.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 03 2012

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