Recombinant Human PKM2 protein (BSA and azide free) (ab178466)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: ELISA, MS, SDS-PAGE, WB
Description
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Product name
Recombinant Human PKM2 protein (BSA and azide free)
See all PKM2 proteins and peptides -
Purity
> 90 % Densitometry. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
MKHHHHHHASKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITA RNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVR TATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKI TLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADF LVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFA SFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMV ARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTR AEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVA RYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNF AMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP -
Predicted molecular weight
59 kDa including tags -
Amino acids
2 to 531 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab178466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Mass Spectrometry
SDS-PAGE
Western blot
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Mass spectrometry
LC-MS/MS -
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.29% Sodium chloride, 0.24% Tris
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ReconstitutionAdd 200 µl of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. ab178466 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
General Info
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Alternative names
- CTHBP
- Cytosolic thyroid hormone binding protein
- Cytosolic thyroid hormone-binding protein
see all -
Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
Tissue specificity
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
Sequence similarities
Belongs to the pyruvate kinase family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab178466 has not yet been referenced specifically in any publications.