Recombinant Human PKM2 protein (BSA and azide free) (ab178466)

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SDS-PAGE - Recombinant Human PKM2 protein (BSA and azide free) (ab178466)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% Densitometry
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag N-Terminus
    • Suitable for: ELISA, MS, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human PKM2 protein (BSA and azide free)
      See all PKM2 proteins and peptides

    • Purity

      > 90 % Densitometry.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P14618

    • Protein length

      Full length protein

    • Animal free

      No

    • Carrier free

      Yes

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MKHHHHHHASKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITA RNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVR TATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKI TLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADF LVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFA SFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMV ARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTR AEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVA RYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNF AMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

      • Predicted molecular weight

        59 kDa including tags

      • Amino acids

        2 to 531

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab178466 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Mass Spectrometry

      SDS-PAGE

      Western blot

    • Mass spectrometry

      LC-MS/MS

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

      Constituents: 0.29% Sodium chloride, 0.24% Tris

    • Reconstitution
      Add 200 µl of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. ab178466 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

    General Info

    • Alternative names

      • CTHBP
      • Cytosolic thyroid hormone binding protein
      • Cytosolic thyroid hormone-binding protein
      • KPYM_HUMAN
      • MGC3932
      • OIP 3
      • OIP-3
      • OIP3
      • OPA interacting protein 3
      • Opa-interacting protein 3
      • p58
      • PK muscle type
      • PK, muscle type
      • PK2
      • PK3
      • PKM
      • PKM2
      • pykm
      • Pyruvate kinase 2/3
      • Pyruvate kinase 3
      • Pyruvate kinase isozymes M1/M2
      • Pyruvate kinase muscle
      • Pyruvate kinase muscle isozyme
      • pyruvate kinase PKM
      • Pyruvate kinase, muscle 2
      • TCB
      • THBP1
      • Thyroid hormone binding protein 1
      • Thyroid hormone binding protein cytosolic
      • Thyroid hormone-binding protein 1
      • Tumor M2 PK
      • Tumor M2-PK
      see all

    • Function

      Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

    • Tissue specificity

      Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

    • Sequence similarities

      Belongs to the pyruvate kinase family.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.

    • Cellular localization

      Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PKM2 protein (BSA and azide free) (ab178466)
      SDS-PAGE - Recombinant Human PKM2 protein (BSA and azide free) (ab178466)

      SDS-PAGE analysis of ab178466

      1) Reduced and heated sample, 2.5 μg/lane

      2) Non-reduced and non-heated sample, 2.5 μg/lane.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab178466? Please let us know so that we can cite the reference in this datasheet.

    ab178466 has not yet been referenced specifically in any publications.

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