Recombinant Human PKM2 protein (BSA and azide free) (ab178466)
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- References
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Overview
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Product nameRecombinant Human PKM2 protein (BSA and azide free)
See all PKM2 proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceMKHHHHHHASKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITA RNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVR TATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKI TLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADF LVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFA SFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMV ARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTR AEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHL QLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVA RYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNF AMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
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Molecular weight59 kDa including tags
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Amino acids2 to 531
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TagsHis tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab178466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Mass Spectrometry
SDS-PAGE
Western blot
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Endotoxin level< 1.000 Eu/µg
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Mass spectrometryLC-MS/MS
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Purity> 90 % Densitometry.
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FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
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ReconstitutionAdd 200 µl of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. ab178466 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
General Info
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Alternative names
- CTHBP
- Cytosolic thyroid hormone binding protein
- Cytosolic thyroid hormone-binding protein
see all -
FunctionGlycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
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Tissue specificitySpecifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
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PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
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Sequence similaritiesBelongs to the pyruvate kinase family.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
- Information by UniProt
Images
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SDS-PAGE - Recombinant Human PKM2 protein (BSA and azide free) (ab178466)SDS-PAGE analysis of ab178466
1) Reduced and heated sample, 2.5 μg/lane
2) Non-reduced and non-heated sample, 2.5 μg/lane.
Datasheets and documents
References
ab178466 has not yet been referenced specifically in any publications.
